Hi Vinay,

For the databases, use .2bit. For your query data, using .fa is fine.

Here is a link to the Blat parameter sets/commands we use for various tasks:
http://genome.ucsc.edu/FAQ/FAQblat#blat5

Happy to help,
Jennifer Jackson
UCSC Genome Bioinformatics Group


Mittal, Vinay K wrote:
> Hi,
> This might be a repeated question for you but it would be great if you can 
> help me here.
> Which database format would you prefer for standalone BLAT? (.fa, .2bit or 
> .nib format)
>
> Thanks..
>
>
> Sincerely,
>   
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