Dear Sirs
I would like to get a copy of all the known genes for a particular
chromosome, extract all the exons and then use this data for another
application. I decided to use the UCSC genes as they are a nice
conservative set of gene predictions for this project.

So using the Table browser, I selected genome: Mouse, assembly: July
2007, groups: Genes and Gene Prediction tracks, track: UCSC genes,
table: known genes, which appeared to have all the information I was
looking for. I then selected region, highlighted position and typed in
chr19. I selected to save the output to a file and I used the ftp site
to select the mouse chromosome sequences. The intention was to get a
copy of all UCSC genes that are annotated as being on Mouse chromosome
19.

When I examined the output from Table browser, I found a number of genes
near the start of the chromosome, however when I looked at this data for
the same build in the Genome Browser, no such genes were shown in the
UCSC genes track. So for example, there are 267 rows of genes from the
Table Browse that are not displayed in the Genome browser. Extracting a
number of these from the corresponding chromosome files, I find the
sequences are primarily Ns, so that does not seem correct.  

My questions are is this the best strategy to go about extracting this
information? Why are there apparently genes located in the first 3
million bases of mouse chromosome 19 with Table Browser but not shown
with Genome Browser? Am I misunderstanding something in setting up the
query?

Thanks
kevin

Kevin Clancy, PhD
Senior Scientist, Informatic Sciences
Invitrogen Corp
Carlsbad, CA 92008
Phone: (768) 268 8356
Email: [email protected] 

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