Hello Yuval, Thank you for alerting about this issue. There were some parameter issues that have since been resolved. They will be incorporated into the next build. Meanwhile, these are our suggested manual changes (very similar the ones you discovered):
Change blatBot.pl near the top of the file, which is currently: my $batchCount = 25; # number of sequences to submit in one request my $sleepTime = 120; # seconds to sleep after request returned To this: my $batchCount = 5; # number of sequences to submit in one request my $sleepTime = 24; # seconds to sleep after request returned Good luck and thank you for your patience while we worked though the solution, Jennifer Jackson UCSC Genome Bioinformatics Group Yuval Itan wrote: > Hello, > > I am trying to use BlatBot.pl for 305 human peptide sequences that I > have. Every time I do it the run is terminated about halfway through > with the message - "did not receive an is_success, dying, content > is:500 Internal Server Error at BlatBot.pl line 112, <FH> line 977". I > was wondering if you have any suggestion of how to solve that, since a > few months ago I used BlatBot.pl and it was just fine. > > Thank you, > > Yuval > > _______________________________________________ > Genome maillist - [email protected] > http://www.soe.ucsc.edu/mailman/listinfo/genome > _______________________________________________ Genome maillist - [email protected] http://www.soe.ucsc.edu/mailman/listinfo/genome
