Hello, Van, typically this is not the kind of question we answer on this list, which is for questions about the Genome Browser. However, see my answers within.
On Sat, 21 Feb 2009 14:29:15 -0500 [email protected] wrote: > Hi There, > Hi There, > > 1) for a known protein sequence, how scientist get the >protein's sequence. > by dna experiment or by protein experiment? these days, typically by DNA seq. > 2) given a gene's sequence and its corresponding protein >sequence (no > mutation marked), > > Do the two sequences completely comply with the >translation rule? yes, although there are exceptions among some Protists, mitochondria and selenocysteines. > if one site in the protein sequence is marked as >mutation, can we know where > the mutation happen in the corresponding gene sequence >and how? > (this is what I most want to know) sometimes. if you know the sequence of the codon that encodes the amino acid in question and the identity of the new AA, often there is only one way to make the conversion in terms of base-pair conversion. You would need to consult the codon tables for each case. best wishes, --b0b kuhn ucsc genome bioinformatics group > Van > > > _______________________________________________ > Genome maillist - [email protected] > http://www.soe.ucsc.edu/mailman/listinfo/genome _______________________________________________ Genome maillist - [email protected] http://www.soe.ucsc.edu/mailman/listinfo/genome
