By the way, normally questions should be addressed to [email protected]
Thanks for the specific info about your machine. Can you also show me example commandlines that you are using to run BLAT? Also, where did you get blat, and where did you get the human chromosome data? BLAT on DNA with -stepSize=5 for extra sensitivity takes about 6GB of RAM for the entire human genome. But a 32-bit compiled blat won't be able to use more than 4GB address space. Thanks! -Galt On Thu, 19 Mar 2009, [email protected] wrote:
? Dear Dr. Galt. ? I'me using USCS Blat standalone version and wants to load and use the interely human genome in my experiments. At the moment, I doing the experiments with isolated human chromosomes, because my computer can't load all the chromosomes in memory. Probably, I done some mistakes in the Blat configuration, because? my computer is good. It is a 64 bits architecture, AMD Atlon X2 dual core processor, with 8 GB-RAM, Linux Fedora 64 bits. Like the input data to Blat program, I using the human genome, in fasta format, that has approximatedely 3GB. How can I? try to run, perfectly, the Blat? ?? Thank you very much. ?? Regards, ????? Herai.
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