This issue is not related to any particular assembly.

 

Can you clarify for me how a PSL line for a query sequence that has
non-contiguous alignments to both the plus and minus strands of the
target. I am referring, specifically, to Example 7 in your on-line
description of how to prepare custom annotation tracks.

 

I have read the information and get the part about how the align block
starts and sizes for the alignments to the minus strand should be
reversed. What's not clear to me is what the final PSL line for this
alignment would look like.

 

First, would the data for the alignments to the plus and minus strands
be combined into a single line of reported on separate lines? If the
data are to be combined, then:

 

1.       What would be reported as the strand?

2.       How would the align block data be combined to report the starts
and sizes?

 

It would be a real help if you could show me what the line (or lines)
for Example 7 (or an equivalent example) would look like.

 

Thanks very much for your help.

 

Regards,

Phil

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