Hi,

OK. I am back with the same request now, after the assembly/annotation 
of Zv8 is done.

We (and also the community, I think) know that, Zv8 was expected as a 
big improvement for Zv7, and it IS indeed, from the analysis report they 
released. So, we eagerly request UCSC, as one of the main hubs of 
data/tool for bioinformatics, to
1. update danRer6 (Zv8) new assembly on UCSC
2. make hg18:danRer6 chain/net alignment
3. put zebrafish self-chain alignment.

Thanks

Regards,

Xianjun


Jennifer Jackson wrote:
> Hello,
> One of our scientists has some specific ideas concerning the zebrafish 
> assembly as follows:
>
> They think the main reason the genome is so difficult to assemble was due
> to the DNA collection strategy:-
> http://www.sanger.ac.uk/Projects/D_rerio/Zv3_assembly_information.shtml
>
> The FAQ indicates there should be a finished genome by the end of this 
> year:
> http://www.sanger.ac.uk/Projects/D_rerio/faqs.shtml#factsnine
>
> Maybe you could discuss your suggestion with the sequencing project,
> and if it would help them, we could discuss it further.
>
> Thank you for your offer to help improve the data,
> Jennifer Jackson
> UCSC Genome Bioinformatics Group
>
> Xianjun Dong wrote:
>> To those who might concern,
>>
>> Zebrafish has been one of the most studied models in study of whole 
>> genome duplication and development, but its genome assembly is not so 
>> well (which is naturally difficult also due to the whole genome 
>> duplication there). We also noticed much duplication closely mapped 
>> in same chromosome, which actually are proved as assembly error in 
>> zv7, by BLATing in the new assembly Zv8 
>> (http://pre.ensembl.org/Danio_rerio/Info/Index). Before Zv8 
>> annotation get done (which might help to some extent, but not all), I 
>> am thinking if UCSC could make a self-chain for zebrafish, just like 
>> you did for human. If that information offered, we could write a 
>> script to quickly check those 'tandem' duplications close in genome, 
>> which can eventually help to improve the quality of the current 
>> assembly.
>>
>> If you think this might not be done in the coming soon by your plan, 
>> I will be appreciated if you can offer any assistance for me to try 
>> it myself.
>>
>> Regards,
>>
>>   

-- 
Sterding (Xianjun) Dong
PhD student, Boris Lenhard's group
Bergen Center of Computational Science
Bergen University, Norway
Mobile: 0047-47361688
Telephone: 0047-55584022
Skype: xianjun.dong 

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