Greetings - Note on probably genome mis-identification of CYP450 in Gallus gallus. It appears a noted CYP4F that occurs within the EST database is more likely a member of the CYP2E2 family.
Name of assemblies (EST Blast): gb|CD735260.1| 4015378 1GAL - Chicken Intestinal Lymphocyte.. gb|BG713654.1| pgl1n.pk010.m8 Normalized Liver Library Gallus.. gb|BU124422.1| 603148196F1 CSEQCHL18 Gallus gallus cDNA clone.. gb|CD763835.1| GGEZLB1006D03.g Limb Bud - LB1 Gallus gallus.. gb|CD764153.1| GGEZLB1010D02.g Limb Bud - LB1 Gallus gallus.. gb|CD731342.1| 4041128 1GAL - Chicken Intestinal Lymphocyte.. And from the following reference: http://drnelson.utmem.edu/chicken.FASTA.htm >New CYP4F family member 63% to 4F22 CD735260 BU124422 CD731342 CD737432found >by Jie Shen, Lei >YangMVLSWLLVALLVALLVAWLRDAVVTVTRFRATCQQLRHFPLPPWRNWLLGHTGMARNTEEGLQDVDKVVAQYRHGCLWWLSPWLPILRLFHPDTLRPVLMASAFTAPKDKLFYGFLKPWLGDGLLLSRGDKWARHRRLLTPAFHFDILKSYVSIFNSSTHIMHTKWRAAAKAVGGPVGRNMMEDLSLLTLDTLQKCIFSHDSHCQEQRSEYIEAILELSTLVVRRQQRLLHHTSWLYRLSADGRRFARACATVHSFTANVVQRRRQELNHLGHQAWLKGRQGRSTDFIDLLLLTKDEDGNTLSDEDISAEADTFMFEGHDTTASGLAWLLYNLAHHPHYQEQCRQEVRELLKGRNVEEIEWEDLSLLPFTTMCIKESLRLHPPVTAVSRRCTEDIAMRDGRVIPKEVICLMSIYGTHHNPDIWPEPQVYDPLRFSPENSQGRSPLAFIPFSAGPRNCIGQSFAMAEMKVVAALTLARFAVRPDPARPVRRKSELILRAEDGLWLQLEPLSAMP* ------------------------------------------------------------ Chromosome position unknown. This CYP450 has probably been misidentified in Gallus gallus since it is most likely it is CYP2E2 and not a 4F family member. I am attaching a dendogram along with this email so you can see that the position the Gallus gallus CYP2E2 appears correct. Protein sequences are provided below for doing a sequence comparison. Thanks, hope this helps, Jim Johnson Prattville, AL The protein sequences below can be used to run a clustal (note that a corrupted polyA insert has been removed from T. guttata). >gi|224154700|ref|XP_002198488.1| PREDICTED: similar to cytochrome P450, family 2, subfamily E, polypeptide 2 homolog [Taeniopygia guttata] MAVATALGSLGAVALGTFAVALLLQWLWNALLAVTRFRATCRQLNKFPIPPWRSWVLGHTGMVRSTEEGL QQVDSLVARYRHGCLWWGLPWLPVLRLFHPSTLRPLLSASAFVAPKDKIFYGFLKPWLGEGLLLSGGERW AQHRRLLTPAFHGDVLRNYLGIFNQSTRVLLAKWGRLAGGGPVELEVLQPLSLLTLDTLQKCIFSHESHC QERPSEYIQAILELSSLVVRRQFRPLLHPWWLYRLSSDGRRFARACATVHNFTADVVQRRRRALTCLGHQ ARLDGHQGHRMDFIDLLLLSKDENGHTLSDEDIAAEAETFMFEGHDTTASGLAWLFYNLAGHPEHQERCR QEVQELLAGRDTADIEWEDLSQLPFTTMCIKESLRLHPPVTAVSRRCTEDIPLRDGRVIPKGVICLMSIY GTHHNPDLWPEPEVFNPLRFSPENSKGRSPSSFIPFSAGPRNCIGQSFAMAEMKVVVALTLSRFVLRRDT ARPPPRRKPELILRAEDGLWLLLEPLLAVP >Proposed CYP4F family member 63% to 4F22 CD735260 BU124422 CD731342 CD737432 found by Jie Shen, Lei Yang [Predicted: Gallus gallus CYP2E2]: MVLSWLLVALLVALLVAWLRDAVVTVTRFRATCQQLRHFPLPPWRNWLLGHTGMARNTEEGLQDVDKVVA QYRHGCLWWLSPWLPILRLFHPDTLRPVLMASAFTAPKDKLFYGFLKPWLGDGLLLSRGDKWARHRRLLT PAFHFDILKSYVSIFNSSTHIMHTKWRAAAKAVGGPVGRNMMEDLSLLTLDTLQKCIFSHDSHCQEQRSE YIEAILELSTLVVRRQQRLLHHTSWLYRLSADGRRFARACATVHSFTANVVQRRRQELNHLGHQAWLKGR QGRSTDFIDLLLLTKDEDGNTLSDEDISAEADTFMFEGHDTTASGLAWLLYNLAHHPHYQEQCRQEVREL LKGRNVEEIEWEDLSLLPFTTMCIKESLRLHPPVTAVSRRCTEDIAMRDGRVIPKEVICLMSIYGTHHNP DIWPEPQVYDPLRFSPENSQGRSPLAFIPFSAGPRNCIGQSFAMAEMKVVAALTLARFAVRPDPARPVRR KSELILRAEDGLWLQLEPLSAMP
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