Hello,

Have you examined the Conservation track? This would be the first choice to 
answer your type of question. Use the Table browser and hg18 to extract regions 
based on human coordinates using web tools.
Link to complete files in Downloads for ftp (see the hg18 track description for 
an explanation of the contents):
http://hgdownload.cse.ucsc.edu/downloads.html#human
scroll to Mar. 2006 (hg18), files under "Conservation" header, to obtain paths

The alternative for a direct mapping of coordinates between species is to use 
the liftOver program. It utilizes the chain track data (as does the net track) 
but is a configurable filter. The best advice is read the usage, try a few 
parameter sets, and evaluate. As you probably know, the syntenic relationship 
is not 1-1 for all cases. For some advice about how scientists here at UCSC 
filter for conservation, an excellent resource would be back in the 
Conservation track's Methodology section.

Link to liftOver web tool: 
http://genome.ucsc.edu/cgi-bin/hgLiftOver

Link to liftOver unix tool binary: 
http://hgdownload.cse.ucsc.edu/downloads.html#source_downloads
http://hgdownload.cse.ucsc.edu/admin/exe/

For the most recent version human->mouse, the file ftp path would be:
goldenPath/hg18/liftOver/hg18ToMm9.over.chain.gz

Thanks,
Jennifer Jackson
UCSC Genome Bioinformatics Group


----- Original Message -----
From: jchl tu <[email protected]>
To: [email protected]
Sent: Tue, 14 Jul 2009 02:53:08 -0700 (PDT)
Subject: [Genome] question about human-mouse genome alignment

HI everyone:
for I have a list of regions in the human genome and want to study if
those regions have conservational counterparts in the mouse genome or
not, I download "mouse net" file from Table Browser and in this file I
found there may be two or even more "top" alignment region spanning
the same regions in the mouse genome.as a result sometimes a same
region in the human genome will have a perfect conservational region
according to one "top" alignment region and may have many gaps
according to another.
my question is which I should trust? and if there exist a better way
to study my problem please tell me (for I want to see detailed
information about alignment say regionA in the human genome may have a
conservational region in the mouse genome but many gaps on it, I want
to see the exact coordinate of the gap and match regions).
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