Hello

 

I have to create the reference file for human genome seq. Do I download
one chromosome at a time or is there a file that contains all?

 

could you please let me know how to create a reference file with
captureed targeted regions in order to use it to align the sequence data
from the Illumina genome analyzer? 

 

I suppose there's a way to do it starting with the target coordinates
and should one add some extra 5'-3'?

 

thanks

 

Valeria

 

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