Hi Dwight,

Yes, it would be best to set up a mirror and then create an assembly for 
S pombe.

Here is some information on mirroring the genome browser:
http://genome.ucsc.edu/admin/mirror.html
http://genomewiki.ucsc.edu/index.php/Browser_Mirrors

If you decide you want a minimal or partial mirror you can read about 
that here:
http://genomewiki.ucsc.edu/index.php/Minimal_Browser_Installation

Here is some information on creating a new assembly:
http://genomewiki.ucsc.edu/index.php/Building_a_new_genome_database

Another helpful resource is the mailing list archive of our 
[email protected] list. You can search the archives from here:
http://genome.ucsc.edu/contacts.html

Katrina Learned
UCSC Genome Bioinformatics Group



Dwight Kuo wrote, On 10/01/10 11:34:
> Hi
>
> Thanks. I was wondering if it's possible to add sequences and annotation for
> S pombe? Would it be easier to build a local installation of the genome
> browser?
>
> Thanks,
>
> D
>
>
>
> On Thu, Sep 30, 2010 at 16:17, Vanessa Kirkup Swing 
> <[email protected]>wrote:
>
>   
>> Dear Dwight,
>>
>> You are using sacCer2 which uses roman numerals for the chromosomes. If you
>> use sacCer1, it works fine. If you go to:
>> http://genome.ucsc.edu/cgi-bin/hgGateway
>>
>> and click on the "sequences" link it will list the chromosome names for the
>> database and assembly you are interested in.
>>
>>
>> Vanessa Kirkup Swing
>> UCSC Genome Bioinformatics Group
>>
>>
>> ----- Original Message -----
>> From: "Dwight Kuo" <[email protected]>
>> To: "Vanessa Kirkup Swing" <[email protected]>
>> Cc: "genome-www" <[email protected]>
>> Sent: Thursday, September 30, 2010 3:53:00 PM GMT -08:00 US/Canada Pacific
>> Subject: Re: UCSC Genome Browser bedGraph format
>>
>> Hi Vanessa,
>>
>>
>> I'm currently inputting:
>>
>>
>>
>> track type=bedGraph
>> chr1 2000 2300 3.0
>>
>>
>> into the S.cereivisae genome from
>> http://genome.ucsc.edu/cgi-bin/hgCustom
>>
>>
>> D
>>
>>
>>
>>
>> On Thu, Sep 30, 2010 at 15:51, Vanessa Kirkup Swing < [email protected]> 
>> wrote:
>>
>>
>> Hi Dwight,
>>
>> Can you tell me what database and assembly you are using? Also, are you
>> using our site: http://genome.ucsc.edu/cgi-bin/hgCustom or are you using a
>> mirror?
>>
>> Thanks!
>>
>>
>> Vanessa Kirkup Swing
>> UCSC Genome Bioinformatics Group
>>
>> ----- Original Message -----
>>
>>
>>
>> From: "Vanessa Kirkup Swing" < [email protected] >
>> To: "Dwight Kuo" < [email protected] >
>> Cc: "genome-www" < [email protected] >
>> Sent: Thursday, September 30, 2010 3:16:21 PM GMT -08:00 US/Canada Pacific
>> Subject: Re: UCSC Genome Browser bedGraph format
>>
>> Hi Dwight,
>>
>> This works for me on on for the human hg18 assembly.
>>
>> Vanessa Kirkup Swing
>> UCSC Genome Bioinformatics Group
>>
>> ----- Original Message -----
>> From: "Dwight Kuo" < [email protected] >
>> To: "Vanessa Kirkup Swing" < [email protected] >
>> Sent: Thursday, September 30, 2010 3:07:16 PM GMT -08:00 US/Canada Pacific
>> Subject: Re: UCSC Genome Browser bedGraph format
>>
>> I tried that before. Actually I'm trying something even simpler:
>>
>>
>>
>> track type=bedGraph
>> chr19 59302000 59302300 -1.0
>> chr19 59302300 59302600 -0.75
>> chr19 59302600 59302900 -0.50
>> chr19 59302900 59303200 -0.25
>> chr19 59303200 59303500 0.0
>> chr19 59303500 59303800 0.25
>> chr19 59303800 59304100 0.50
>> chr19 59304100 59304400 0.75
>> chr19 59304400 59304700 1.00
>>
>>
>>
>> On Thu, Sep 30, 2010 at 14:59, Vanessa Kirkup Swing < [email protected]> 
>> wrote:
>>
>>
>> Dear Dwight,
>>
>> The example that you are referring to is from this page:
>>
>> http://genome.ucsc.edu/goldenPath/help/bedgraph.html
>>
>> There is a note that says "if you copy/paste the above example, you have to
>> remove the carriage returns."
>>
>> If you do this, it should work.
>>
>> Vanessa Kirkup Swing
>> UCSC Genome Bioinformatics Group
>>
>>
>>
>>
>>
>> ----- Original Message -----
>> From: "Dwight Kuo" < [email protected] >
>> To: [email protected]
>> Sent: Thursday, September 30, 2010 1:39:16 PM GMT -08:00 US/Canada Pacific
>> Subject: UCSC Genome Browser bedGraph format
>>
>> When I try to input the UCSC Genome Browser's examples for bedGraph format,
>> i.e.:
>>
>>
>>
>> browser position chr19:59302001-59304701
>> browser hide all
>> browser pack refGene encodeRegions
>> browser full altGraph
>> # 300 base wide bar graph, autoScale is on by default == graphing
>> # limits will dynamically change to always show full range of data
>> # in viewing window, priority = 20 positions this as the second graph
>> # Note, zero-relative, half-open coordinate system in use for bedGraph
>> format
>> track type=bedGraph name="BedGraph Format" description="BedGraph format"
>> visibility=full color=200,100,0 altColor=0,100,200 priority=20
>> chr19 59302000 59302300 -1.0
>> chr19 59302300 59302600 -0.75
>> chr19 59302600 59302900 -0.50
>> chr19 59302900 59303200 -0.25
>> chr19 59303200 59303500 0.0
>> chr19 59303500 59303800 0.25
>> chr19 59303800 59304100 0.50
>> chr19 59304100 59304400 0.75
>> chr19 59304400 59304700 1.00
>>
>>
>> This fails, with the message "Error Unrecognized format type=bedGraph line
>> 10 of custom track"
>>
>>
>> Sincerely,
>>
>>
>> Dwight
>>
>>     
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