Respected sir/madam, I have taken the data of Refseq genes of Rat(organism) from the Table browser and now I want to use txStrt coordinates to find the coordinates for the region 5000 above the transcription start site. In case of chromosome with + strand I am subtracting from the given txtStrt coordinate but in case of chromosome with - strand, how should I go about it, should I subtract 5000 or should I add 5000, actually I want to focus on the promoter region usually present upstream from the transcription site.
Also how should I get the 5' UTR and 3'UTR using the coordinates of coding region position and transcription position. Thanks in advance With regards, Vaibhav Jain Research Scholar @IGIB New Delhi, INDIA _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
