Good Morning Vaibhav:

To calculate GC percent over regions greater than 5 bases, you need
to use the kent source tree program: hgGcPercent

Fetch the kent source tree and build this program.  You can then calculate
any window size GC percent from the .2bit file.  The program
is in the directory: src/hg/makeDb/hgGcPercent/

See also: http://genomewiki.ucsc.edu/index.php/Wiggle

--Hiram

vaibhav jain wrote:
> Hello everyone,
>  I want to download the GC percent of Rat genome, when I am trying from
> Table browser I am getting data in 5 bp window and that too only of one
> chromosome at a time. Now I want the GC percent in much higher window
> atleast 1 kb, so is there any tool (in UCSC/Galaxy) through which I can get
> the data in 1 kb or higher window . Also when I downloaded the data from
> download page then I could not see any kind of percentage or I am not able
> to interpret the data in proper manner. This how the file looks, some of the
> starting lines of the files are:
> 
> 585 chr1 0 5120 chr1.0 5 1024 0 /gbdb/rn4/wib/gc5Base.wib 0 100 781 32475
> 1671220
> 585 chr1 5120 10240 chr1.1 5 1024 1024 /gbdb/rn4/wib/gc5Base.wib 0 100 1009
> 33205 1516480
...
> 589 chr1 525310 530430 chr1.99 5 1024 98233 /gbdb/rn4/wib/gc5Base.wib 0 100
> 1024 41420 2149200
> 
> Thanks and regards
> Vaibhav Jain
> Research Scholar @IGIB
> New Delhi, INDIA
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