Hi Heater: If you just need highlighting of two pairs of primers, you can try this idea:
click PCR, input your first primers, look at result, click DNA, submit, copy paste sequence into blat, select best match. Then click PCR, input your second pair of primers. -> Both sequences will be shown. I've mentioned this before on http://www.mail-archive.com/[email protected]/msg03410.html but the question wasn't on primers at the time, so I didn't realize immediately that your question was similar. cheers Max On Wed, Feb 16, 2011 at 7:49 PM, Galt Barber <[email protected]> wrote: > Hi, Heather > > hgPcr has no facility for doing more than one PCR result at a time. > > You can however use the standalone commandline utility isPcr that > comes with BLAT to query multiple primer pairs at once. > I recommend using the psl output with this option: -out=psl > > Then you can make a custom track from your psl output > and visualize it in the browser. > > -Galt > > On 02/16/11 09:06, Heather Miller wrote: >> Hello, >> I am using the March 2006 human assembly (RefSeq genes) to view PCR products >> of CstF3 using in-silico PCR. I am just wondering if it is possible to have >> more than one PCR search displayed at once. For example, I want to view >> where two differently sized products using two different reverse primers >> would span. >> >> Thank you, >> Heather Miller >> >> >> Heather Miller, PhD >> Teaching Associate >> North Carolina State University >> Biotechnology Education Facility >> 6105 Jordan Hall >> Box 7512 >> Raleigh, NC 27695 >> [email protected] >> (919) 513-7685 >> www.ncsu.edu/biotechnology >> _______________________________________________ >> Genome maillist - [email protected] >> https://lists.soe.ucsc.edu/mailman/listinfo/genome > _______________________________________________ > Genome maillist - [email protected] > https://lists.soe.ucsc.edu/mailman/listinfo/genome > _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
