Good Morning Ravi:  Your best option would probably be to download
the blat binary and .2bit file for the genome you want to use and
run your blat operations locally.
http://genome-test.cse.ucsc.edu/~kent/exe/
http://genome.ucsc.edu/goldenPath/help/blatSpec.html
http://hgdownload.cse.ucsc.edu/gbdb/hg19/

--Hiram

----- Original Message -----
From: "Ravi Subramanian" <[email protected]>
To: [email protected]
Sent: Friday, March 25, 2011 10:12:02 AM
Subject: [Genome] Large BLAT queries

Hello,
  My name is Ravi Subramanian, I am a postdoc at Tufts University, and I am
trying to do a comparative genomics study, where I would need to do a batch
BLAT search of upwards of 200-300 sequences at a time (each sequence is
approximately 3 kb).  Is there a way I can do this without the limitations
of the standard BLAT interface?  Thanks,

Ravi

-- 
Ravi Subramanian, Ph.D.
Postdoctoral Associate
Tufts University
136 Harrison Ave.
Boston, MA 02140
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