Hi Hiram,

In that case, is using chromFaMasked.tar.gz more straightforward? What
is the difference between chromFaMasked.tar.gz and the fasta file
converted from mm9.2bit?

On Tue, Mar 29, 2011 at 10:03 AM, Hiram Clawson <[email protected]> wrote:
> Please use the twoBitToFa program to extract sequence from .2bit files.
>
> --Hiram
>
> ----- Original Message -----
> From: "Peng Yu" <[email protected]>
> To: "Hiram Clawson" <[email protected]>
> Cc: [email protected]
> Sent: Tuesday, March 29, 2011 6:38:36 AM
> Subject: Re: [Genome] Perl Bio::Das download of repeatmasked sequences?
>
> Hi Hiram,
>
> I know that uscs browser source code should have a parer to this type
> of files (which is in C (according to the accompany header file),
> right?). Is there a library as simple as Bio::DAS that I can call from
> scripting language like perl or python without having to know the
> details of .2bit file format?
>
> http://www.perlmonks.org/?node_id=672251
>
> Converting .2bit to fasta then using bioperl to parse fasta is one
> option. But it seems less straightforward. If there is a stand alone
> tool, it would be better.
>
> On Mon, Mar 28, 2011 at 7:33 PM, Hiram Clawson <[email protected]> wrote:
>> Please use the .2bit files fetched from hgdownload for
>> local access to genome sequence.
>>
>> --Hiram
>>
>>
>> On 3/28/11 9:19 AM, Peng Yu wrote:
>>> Hi,
>>>
>>> I'm wondering whether ucsc genome browser DAS has enabled the support
>>> repeatmasked sequences. I know that it was not available, but I want
>>> to double check if it is available now.
>>>
>>> https://lists.soe.ucsc.edu/pipermail/genome/2007-March/013165.html
>>
>
>
>
> --
> Regards,
> Peng
>



-- 
Regards,
Peng
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