Hi, The lab I am supporting would like to link our web content repository to UCSC BLAT or NCBI BLAST web functions. It is an internal web so the usage will be quite light, couple times a day at most, looking up oligo sequence by creating these oligo sequences into html links, setting the query parameter.
Our preference is to use BLAT, so much faster and the nicety of getting the results on the genome browser. Also the insillico PCR function. As the BLAT web ui loads, I see that I can construct the organism, and sub db preference within the url/browser links: ie loading C. elegans and db ce4 http://genome.ucsc.edu/cgi-bin/hgBlat?org=C.%20elegans&db=ce4 But when I try passing in a sequence, looks to be userSeq http://genome.ucsc.edu/cgi-bin/hgBlat?command=start&org=C.%20elegans&db=ce4&userSeq=AATTAATTAATTAATTAATT Here is the output from webpage: Sorry, didn't find input variable type Probably the web page didn't mean to call this program. Am I doing something silly? Looks like the insillico PCR allows passing in sequence, as does the BLAST. Thanks, Jake -- jl _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
