Here is some information on the BLAT PSL format: http://genome.ucsc.edu/goldenPath/help/hgTracksHelp.html#PSL
The first strand character (+) refers to the query, the second (-) refers to the genome (or database target). So in this case it means that the (not-reverse-complemented) query was aligned to the reverse-complemented target database. -Galt 7/5/2011 1:16 PM, Bhagwat, Medha (NIH/OD/ORS) [E]: > HI, > > I am trying to understand the BLAT output for a protein query search. What > does the COLUMN strand reported as +- mean? > > Thanks, > > Medha > > > _______________________________________________ > Genome maillist - [email protected] > https://lists.soe.ucsc.edu/mailman/listinfo/genome _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
