Dear UCSC team,

below i provide my previous question and the answer from your colleague to 
avoid 
confusion in future. So once more, how can I make a custom track from fasta 
file 
containing cca. 100.000 sequences? Blat allows only 25 per time. Maybe you have 
scripts for that?

Thanks,

Mitja



Hi Mitja,

You will want to use Blat to map your fasta sequence to the genome and
then generate the output PSL format. The PSL format can the be
uploaded as a custom track.

To do this click on "Blat" from the blue navigation bar. Select the
appropriate Genome,Assembly,Query type, Sort output, and select "PSL"
for the Output type.

Once you have the PSL formatted output you can upload your custom
track by going to the gateway page
(http://genome.ucsc.edu/cgi-bin/hgGateway) and clicking on "custom
tracks".

If you have further questions, please contact the mailing list:
[email protected].

Vanessa Kirkup Swing
UCSC Genome Bioinformatics Group



---------- Forwarded message ----------
From: Mitja Mitrovic <[email protected]>
Date: Thu, Jul 21, 2011 at 9:16 AM
Subject: [Genome] New ncRNA track
To: [email protected]


Dear UCSC team,

I have a fasta file of approx. 100.000 sequences (up to 50 bp in length). I
would like to create a custom track with those sequences to see where they fall
in the genome. Therefore I would need a ped file with bp position ect. So, my
question is how can i make a custom track from this fasta file? Thanks in
advance!

Mitja
********************************************************
Mitja Mitrovic
PhD Student
Department of Genetics
Oostersingel, Ingang 47
9713 EX Groningen
The Netherlands
2nd floor, room E2.047
Phone:+31(0)50-3617-105
53°15'N 06°35'E
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