Hi, I'm having difficulty displaying a bigWig custom track from a url on the browser. here's what I've done:
File creation: bowtie -m 1 -S --best --strata -q -p 8 --un no_align/file.fastq --max mult_align/file.mult.fastq bowtie/indexes/hg19 file.fastq align/file.sam samtools view -b -S align/file.sam -o align/file.bam genomeCoverageBed -bg -ibam align/file.bam -g /genomes/hg19.sizes > align/file.bedgraph (final bedgraph has no header/header removed) wigToBigWig align/file.bedgraph /genomes/hg19.sizes align/file.bw Visualisation: I gave the full url address of the bigwig file As per http://genome.ucsc.edu/goldenPath/help/bigWig.html example three. I then, firstly left out the optional documentation which gave the error: "Error Unrecognized format line 1..." when using "Add Custom Tracks" I then added the track documentation as given in the example above at point 7* *track type=bigWig name="SL2901" description="test" bigDataUrl= http://maniatis.c2b2.columbia.edu/Noam/SL2901.bw However this resulted in the following error*: Error Optional track documentation appears to be either too large (greater than 50k) or it starts with a track or browser line. This is usually an indication that the data has been accidentally put into the documentation field. Only html documentation is intended for this field. Please correct and re-submit. *The url address is valid and public so should be accessible. Any help would be great, Thanks, Sean* * _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
