Dear Katrina


Thank you for the info.

Not clear how nucleotide numbering is handled for gaps and chromosome ends when 
one uses the entire chromosomes to create bed files.

How could one check for quality control?

Minou Bina 



----- Original Message -----
From: "Katrina Learned" <[email protected]>
To: "Minou Bina" <[email protected]>
Cc: [email protected]
Sent: Friday, September 16, 2011 7:11:18 PM
Subject: Re: [Genome] bed files  for entire chromosomes

Hi Minou, 

Here is some information about creating bed files: 
http://genome.ucsc.edu/FAQ/FAQformat.html#format1 

You might consider converting your bed files to our bigBed format using the 
program bedToBigBed . The main advantage of the bigBed files is that only the 
portions of the files needed to display a particular region are transferred to 
UCSC, so for large data sets bigBed is considerably faster than regular bed 
files. The bigBed file remains on your web accessible server (http, https, or 
ftp), not on the UCSC server. 

I am not sure if this is what you were asking, but to specify a block to cover 
the entire chromosome, you would set your chromStart to 0 and your chromEnd to 
the length of the chromosome. To find out the length of each chromosome, from 
the gateway page ( http://genome.ucsc.edu/cgi-bin/hgGateway ), use the 
drop-down menus to select your assembly of interest, and under the drop-downs, 
in the light blue bar that states, " About the <assembly information> 
(sequences)", click on the 'sequences' link. For example, the hg19 sequences 
link would take you here: 
http://genome.ucsc.edu/cgi-bin/hgTracks?db=hg19&chromInfoPage= 

I hope this information is helpful. Please contact the mail list ( 
[email protected] ) again if you have any further questions. 

Katrina Learned 
UCSC Genome Bioinformatics Group 

On 9/16/11 12:58 PM, Minou Bina wrote: 

Hi

We are planning to analyze entire human chromosomes and create .bed files for 
display on the human genome browser.

It is clear how we should specify the block position for an entire chromosome 
and how we should do the numbering.

Minou Bina
Purdue University
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