Dear UCSC Genomics, I've been trying to convert a ~195MB fixedStep wig file to bigwig, using wigToBigWig, but keep coming up with this error: 'There's more than one value for chr1 base 40730 (in coordinates that start with 1).'
For my fixedStep files, I'm using the following header: fixedStep chrom=chr1 start=10010 step=30 span=300. The data is continuous for each chromosome, ie every step along the chromosome has a score of >= 0, without exceeding the length of the chromosome. When I load the data for a single chromosome, or a group of chromosomes in wig format directly to the UCSC browser, the data is displayed correctly, and matches my pile-up track perfectly. When I try to convert a single chromosome wig file to bigWig, I get the same error. When I look at my data around the base position that is called as being in error, it is typically in an area with scores of many 0's either side, but seems to me otherwise unremarkable. It isn't occurring near a fixedStep title line. I've tried adjusting my bin sizes and span sizes, my start positions etc, but to no avail. I don't fully understand exactly what the error means, which makes it much more difficult to fix! If anyone could explain what causes this type of error, I would be very grateful! Thanks in advance, Michael McCarthy, Wellcome Trust Centre for Cellular and Molecular Physiology, University of Oxford, UK _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
