Thanks for the update Jaaved!

- Greg

On 11/14/11 8:04 AM, Jaaved Mohammed wrote:
> Hi all,
>
>
>
> The folk on the Flybase help mailing list were able to answer my questions
> regarding the persimilis and willistoni scaffolding naming discrepancies
> between the UCSC genome browser and Flybase:
>
>
>
> 1.       D. persimilis:
>
> There is a one-to-one relationship between the UCSC "scaffold_" prefixed
> headers and the "super_" prefixed headers. For example, scaffold_14
> corresponds to super_14.
>
>
>
> 2.       D. willistoni:
>
> The mapping is not so trivial as it is for persimilis. Both UCSC genome
> browser and Flybase uses the AAA CAF1 assemblies produced in 2007. However,
> 89 scaffolds from the CAF1 assembly for Dwil were later suppressed from the
> CAF1 assembly before it was added to GenBank because these 89 scaffolds
> mapped to Wolbachia. The scaffold IDs were also changed to avoid confusion
> with the previous (original) assembly. Explicitly, it appears that UCSC
> genome browser is using the unsuppressed, original willistoni assembly and
> Flybase is using the modified assembly.
>
>
>
> A mapping between the original AAA version and what exists in
> GenBank/FlyBase can be found here:
>
> ftp://ftp.ncbi.nih.gov/genomes/Drosophila_melanogaster/special_requests/CAF1
> /dwil/dwil_scaffold2GenBank
>
>
>
> A full list of problematic scaffolds that were found during the GenBank
> submission process (for all 12 Drosophila genomes) can be found here:
>
> ftp://ftp.ncbi.nih.gov/genomes/Drosophila_melanogaster/special_requests/CAF1
> /foreign_scaffolds_in_caf1.txt
>
>
>
>
>
>
>
>
>
> This explanation has been reworded from the response I received on the
> Flybase help mailing list. I thank everyone for their generous help with
> this ambiguity. Hopefully others who encounter  this discrepancy will find a
> suitable answer in this thread.
>
>
>
> Thanks,
>
> Jaaved
>
>
>
> --
> Jaaved Mohammed,
> Ph.D. Student of Computational Biology
> Tri-Institutional Training Program in Computational Biology and Medicine
> (Cornell University - Ithaca, Weill Cornell Medical College, and Memorial
> Sloan-Kettering Cancer Center)
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