Thanks, Luvina.

>From trial and error on a server with 16Gb of RAM, I was able to build a 
>database of 7.5 million 100 bp sequences.  As you mentioned the limit I ran 
>into was the final size of the database (based on the input file), and not the 
>number of sequences.

Vince

________________________________________
From: Luvina Guruvadoo [[email protected]]
Sent: Tuesday, November 22, 2011 11:16 AM
To: Vince Forgetta
Cc: [email protected]
Subject: Re: [Genome] max of seqCount in 2bit

Hi Vince,

This format supports at least 100 million sequences, but the maximum
varies based on the input file. Please contact us again at
[email protected] if you have any further questions.

---
Luvina Guruvadoo
UCSC Genome Bioinformatics Group


On 11/17/2011 6:30 PM, Vince Forgetta wrote:
> Hi,
>
> I am curious as to the maximum number of sequences allowed within a 2bit file 
> (keeping the total sequence content within the 4Gb limit).  Is the count 
> stored as a 32-bit integer? From 
> http://genome-source.cse.ucsc.edu/gitweb/?p=kent.git;a=blob_plain;f=src/inc/twoBit.h;hb=HEAD
>  it would appear as such.
>
> Thanks in advance for the help.
>
> Vince
> _______________________________________________
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