Hi Edoardo,

We suggest that you skip this step:

1. I've clicked on "Choose file" button and selected the file from my
computer

The file will be accessed from the URL in the custom track definition so no
uploading is necessary.

If you have further questions, please write to the mailing list:
[email protected].

Vanessa Kirkup Swing
UCSC Genome Bioinformatics Group

---------- Forwarded message ----------
From: Edoardo Giacopuzzi <[email protected]>
Date: Tue, Dec 20, 2011 at 7:16 AM
Subject: [Genome] Adding custom track in vcf format
To: [email protected]


Hi,

I want to add a custom track containing information from a vcf file
downloaded from the Exome Variant Server
(http://evs.gs.washington.edu/EVS/). I've downloaded the file in bgzip
format and indexed it using tabix, according to instruction on the VCF
help page from Genome browser
(http://genome.ucsc.edu/goldenPath/help/vcf.html).

Then I've opened the add Custom Track page in Genome Browser and did as
following:
1. I've clicked on "Choose file" button and selected the file from my
computer
2. In the text box I've added this custom track definition

track type=vcfTabix name="NEU1_EVS"
bigDataUrl=http://myorg.edu/mylab/EVS_SNP_NEU1.vcf.gz description="NEU1
variants from EVS project" visibility=pack db=hg19 chromosomes=chr6

However the system use to show this error:

 Error File 'EVS_SNP_NEU1.vcf.gz' - Unrecognized format line 31 of
somefile.gz: 6 31827505 rs114618932 C T . PASS
DBSNP=dbSNP_132;EA_AC=0,4488;AA_AC=7,3027;TAC=7,7515;MAF=0.0,0.2307,0.0931;GTS=TT,TC,CC;EA_GTC=0,0,2244;AA_GTC=1,5,1511;GTC=1,5,3755;DP=121;GL=NEU1;CP=0.0060;CG=-3.45;AA=C;CA=unknown;EXOME_CHIP=no;GM=NM_000434.3;FG=coding-synonymous;AAC=none;PP=413/416;CDP=1239;GS=NA;PH=unknown
(note: chrom names are case sensitive)

I guess that it would be something wrong in the upload process or custom
track definition, since I obtain the same error using data and track
definition from Genome Browser tutorial example (Example Two from the
help page http://genome.ucsc.edu/goldenPath/help/vcf.html).

Any suggestion?
Thanks!

Edoardo Giacopuzzi, Ph.D.
_______________________________________________
Genome maillist  -  [email protected]
https://lists.soe.ucsc.edu/mailman/listinfo/genome
_______________________________________________
Genome maillist  -  [email protected]
https://lists.soe.ucsc.edu/mailman/listinfo/genome

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