Hi Deli,

We recommend using bigBed. We have a utility called bedToBigBed which can
be used for the conversion. More information on bigBeds and the bedToBigBed
utility can be found here:

http://genome.ucsc.edu/goldenPath/help/bigBed.html

If you have further questions, please email the list: [email protected].

Vanessa Kirkup Swing
UCSC Genome Bioinformatics Group


---------- Forwarded message ----------
From: Deli Liu <[email protected]>
Date: Tue, Dec 20, 2011 at 8:34 AM
Subject: Re: [Genome] xenoRefGene Help
To: [email protected]


Hi

I am trying to upload RNA-seq results into the UCSC genome browser in
http://www.genome.ucsc.edu/cgi-bin/hgCustom. And I make my input format
same as * *ENCODE RNA elements: BED6 + 3 scores format, but I still cannot
upload the data by specifying the track type:

# Displaying first 50 lines of data
track type='BED6+3'
chr1    3241624    3242705    CUFF.1.1    1000    +    1.1704528930
0.05    2.552442
chr1    3243860    3244536    CUFF.2.1    1000    +    1.2850250455
0.05    2.714722
chr1    3363571    3363757    CUFF.3.1    1000    +    19.6351241782
0.05    23.244555
chr1    3363904    3363988    CUFF.4.1    1000    +    719.2170299779
0.05    784.209311

It seems I have the problem in the track type. So which track type I should
use to upload my RNA-seq results with fpkm value and reads density?

Thanks a lot.

Best

On Wed, Jun 29, 2011 at 4:00 PM, Deli Liu <[email protected]> wrote:

> What does exonFrames (-1, 1, 0, 1 and 2 in last column) mean in the
> xenoRefGene of dog canFam2?
>
> NM_020923 chr37 + 17871328 17896391 17880435 17893285 40
>
17871328,17880435,17886560,17887180,17887966,17888306,17888578,17888815,17889124,17889261,17889288,1
>
>
7889647,17889913,17890291,17891753,17892167,17892439,17892654,17893060,17893874,17894018,17894082,17894182,17894353,17894462,17894608,17894762,17894806,17894
>
847,17894887,17894948,17895118,17895149,17895258,17895360,17895416,17895514,17895853,17895939,17896032,
> 17871373,17880582,17887158,17887943,17888209,17888567
>
>
,17888806,17889111,17889131,17889277,17889396,17889677,17890274,17891509,17892139,17892286,17892571,17892687,17893285,17893966,17894044,17894099,17894191,178
>
94403,17894576,17894662,17894780,17894825,17894873,17894902,17895041,17895136,17895238,17895321,17895396,17895467,17895554,17895898,17896023,17896391,
> 0 ZDBF
> 2 incmpl incmpl
>
-1,1,1,0,0,1,0,0,0,2,2,1,0,0,1,1,0,2,0,-1,-1,-1,-1,-1,-1,-1,-1,-1,-1,-1,-1,-1,-1,-1,-1,-1,-1,-1,-1,-1,
>
> Thanks a lot
>
> --
> Deli Liu
> Institute of Bioinformatics, University of Georgia
> Athens, GA, 30605
>



--
Deli Liu
Institute of Bioinformatics, University of Georgia
Athens, GA, 30605
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