I think the issue may be with the bigBED format files we use in our search 
function. I am using the BED files which are in BED Details format, and using 
the bedToBigBed program to generate the bigBED files. The command I am using is 
(example):

grep -v track bed_detail.bed | sort -k1,1 -k2,2n > /tmp/tmp_bed_detail.bed
bin/bedToBigBed -tabs -bedFields=9 -as=ISCA.as /tmp/tmp_bed_detail.bed 
hg18.chrom.sizes big_bed_detail.bb

where ISCA.as is:

table nstd37CustomTracks
"ISCA Custom Tracks with NCBI variant ID and variant details link"
(
string  chrom;      "Reference sequence chromosome or scaffold"
uint    chromStart; "Feature start position"
uint    chromEnd;   "Feature end position"
string  name;       "CNV title"
uint    score;      "Score"
char[1] strand;     "+ or - for strand"
uint    thickStart; "Color start position"
uint    thickEnd;   "Color end position"
string    itemRGB;    "Blue for duplication, Red for deletion"
string  variantID;  "NCBI variant ID"
string  variantDetails;      "html1"
)

The output of bedToBigBed run on the ISCA_Uncertain track is shown:

pass1 - making usageList (24 chroms): 2 millis
pass2 - checking and writing primary data (2791 records, 11 fields): 20 millis
index write: 0 millis
pass3 - writeReducedOnceReturnReducedTwice: 5 millis
further reductions: 2 millis

I have attached an image of the error we are getting.

Viren
________________________________
From: Vanessa Kirkup Swing [[email protected]]
Sent: Tuesday, December 20, 2011 8:12 PM
To: Patel, Viren
Cc: [email protected]
Subject: Re: [Genome] Error with ISCA custom tracks

Hi Viren,

We are unable to reproduce what you are describing. Can you please tell us all 
of the steps that led you to the Invalid signed integer error?

Thanks!

Vanessa Kirkup Swing
UCSC Genome Bioinformatics Group

---------- Forwarded message ----------
From: Patel, Viren <[email protected]<mailto:[email protected]>>
Date: Tue, Dec 20, 2011 at 4:30 PM
Subject: [Genome] Error with ISCA custom tracks
To: "[email protected]<mailto:[email protected]>" 
<[email protected]<mailto:[email protected]>>


Hello. We have previously generated custom tracks for ISCA CNV data 
(https://www.iscaconsortium.org/index.php/the-news/139-new-ucsc-tracks-now-available).
 Recently the Genome Browser has started giving an "Invalid signed integer" 
error when clicking on a CNV. We have not had any changes to the tracks and I 
am wondering if something may have changed in the Genome Browser custom tracks 
requirements. Please let me know what additional data you may need and how I 
can troubleshoot this issue. Thanks.

Viren Patel
www.iscaconsortium.org<http://www.iscaconsortium.org>

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