Thank you Greg,

I added start+1 and stop+1 to all my gtf entries and now I don’t get that error.

Abi


On 1/25/12 3:10 PM, "Greg Roe" <[email protected]> wrote:

  Hi Abi,

  Negative one is an illegal value for a chromStart. GTF files are 1-relative 
coordinates where the first base of a chromosome is 1, the equivalent 
chromStart value is 0. See also: 
http://genome.ucsc.edu/FAQ/FAQformat.html#format4

 Please let us know if you have any additional questions: [email protected]

 -
 Greg Roe
 UCSC Genome Bioinformatics Group

 On 1/23/12 12:58 PM, Lakshmanan, Abirami wrote:

Hi,

I am trying to upload a gtf file (output from cufflinks/cuffmerge of ucsc 
refseq zv9 and ucsc ensembl gene  zv9) as a custom track to the UCSC site and I 
keep getting this following error.

  Error track load error (track name='ct_UserTrack_3545'):
start -1, end 3618 out of range in findBin (max is 512M)

I tried removing the entries which start at 1, 0 and are on the negative strand 
and still I get the same error.
Has anyone encountered this before?
Thanks for the help.

Abi

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