Hi Rita,

Can you tell us in detail the problems that you are seeing and the
steps to reproduce them? If you could send this to:
[email protected], that would be great.

Thanks!

Vanessa Kirkup Swing
UCSC Genome Bioinformatics Group


---------- Forwarded message ----------
From: Rita Rebollo <[email protected]>
Date: Fri, Feb 3, 2012 at 10:44 AM
Subject: [Genome] Encode wig files with cistrome
To: "[email protected]" <[email protected]>


Hello, I hope this is the right place to ask this question. I m trying
to use part of the mouse Encode data through cistrome/galaxy. I m
using the UCSC Main table browser to get the file to Cistrome. I have
chosen mouse, expression and regulation, LICR TFBS or Histones, and
the "signal files" that are supposed to be wig files. Nevertheless,
the WIG files are not working with some of the tools available in
Cistrome (as SitePro) for instance. So I m wondering if the wig files
are correct. This is what I see :

track type=wiggle_0 name="ES-Bruce4 H3K4me1" description="ES-Bruce4
H3K4me1 Histone Modifications by ChIP-seq Signal from ENCODE/LICR"
chr1    3002700 3002800 0.17
chr1    3002800 3002900 0.17
chr1    3002900 3003000 0.17
chr1    3004200 3004300 0.13
chr1    3010800 3010900 0.13
chr1    3011000 3011100 0.17
chr1    3011100 3011200 0.13
chr1    3016200 3016300 0.17
chr1    3016300 3016400 0.17
chr1    3016400 3016500 0.21
chr1    3016500 3016600 0.25
chr1    3016600 3016700 0.17
chr1    3016700 3016800 0.13
chr1    3025500 3025600 0.13
chr1    3025600 3025700 0.13
chr1    3025700 3025800 0.25
chr1    3025800 3025900 0.21
chr1    3025900 3026000 0.29

for 9 000 000 lines of course.


Any idea of what is happening?

Thank you

Dr. Rita Rebollo
BCCRC Terry Fox Laboratory
[email protected]

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