Dear Greg,
Thanks for your reply. I think I didn't make it clear in my previous email. I 
am not looking to get the sequence. I am looking for the alignment of a refseq 
sequence and the chromosome sequence from hg19. Is there a way to get the 
alignment in a text format like  by clicking "Side by Side Alignment" in the 
left panel in the link I sent:
http://genome.ucsc.edu/cgi-bin/hgc?g=htcCdnaAli&i=NM_000022&c=chr20&l=43248162&r=43280376&o=43248162&aliTable=refSeqAli&table=refGene
Regards,
Xiaomao

-----Original Message-----
From: Greg Roe [mailto:[email protected]] 
Sent: Tuesday, February 21, 2012 4:08 PM
To: Xiaomao Zhu
Cc: [email protected]
Subject: Re: [Genome] question on how to get the alignment of a refseq gene to 
genomic sequence

Hi Xiaomao,

You can get that using the table browser: 
http://genome.ucsc.edu/cgi-bin/hgTables

Select hg19 then...
group: Gene and Gene Pred Tracks
track: RefSeq Genes
table: refGene
region: genome

Next to identifiers click the paste list button, and paste in your list (i.e., 
NM_000022).

Then select "sequence" for your output.

- Greg


On 2/20/12 12:47 PM, Xiaomao Zhu wrote:
> Hi,
> I'm interested in getting a text output format of the alignment of a refseq 
> gene (i.e., NM_000022) to hg19 genomic sequences, something like:
>
> http://genome.ucsc.edu/cgi-bin/hgc?g=htcCdnaAli&i=NM_000022&c=chr20&l=
> 43248162&r=43280376&o=43248162&aliTable=refSeqAli&table=refGene
>
> Do you provide a tool or API to achieve that?
>
> Thanks.
>
> Xiaomao Zhu
>
> _______________________________________________
> Genome maillist  -  [email protected] 
> https://lists.soe.ucsc.edu/mailman/listinfo/genome

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