Hi

I had a question regarding the knownCanonical transcripts which we can find 
from  the UCSC table browser.
Are these the transcripts with all the coding exons of the gene or the 
transcript with the longest CDS based on experimental evidence ?

I assume it to be the latter as not all genes have a canonical transcript ID 
associated with them.

Also how should one choose a single transcript for a gene for annotation 
purposes as Canonical transcript might not be the most important / most studied 
 / most expressed transcript ? For example many publications use the 
NM_001203247 transcript for annotating EZH2 mutations (like Y641F) while 
according to Canonical transcript (and Cosmic which probably uses Canonical 
transcrpt) it should be NM_004456 (and thus the mutation should be Y646F as in 
Cosmic)

Could you please clarify my doubts.

Thanks & Regards
--
Rahul



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