Good Morning Yec'han: It appears that your MIRA assembler software is constructing a wiggle file that defines data points outside of your sequence. The wigToBigWig is correctly interpreting the step size of 4 in the wiggle file, but it is running out of sequence length to complete the last data point. You should check your software that produces the wiggle file. wigToBigWig is correctly reporting the error.
--Hiram Yec'han Laizet wrote: > Hello, > > I used the wigToBigWig together with Galaxy to view a custom track. The wig > file came from an assembly with the MIRA assembler. It contains the read > coverage. > > When trying to use wigToBigWig, it complained about a length difference. I > figured out that it was because of the step value was set to 4 in the wig > file. > As 4 is not a multiple of my sequence length, I got this error. > > A workaround was to add undefined bases at the end of the sequence to make > its > length being a multiple of 4. It would be easier if wigToBigWig takes in > account the step value in the wig file. > > Best regards. > > Yec'han _______________________________________________ Genome maillist - [email protected] https://lists.soe.ucsc.edu/mailman/listinfo/genome
