Dear supporter,

1.       I would like to ask whether the BED detail format (which includes 2 
additional columns) can be transformed into the bigBED format? When I try to 
transform BED detail format to bigBED using the function bedToBigBed it fails.

I get this error:

./bedToBigBed test_bb.BED hg19.chrom.sizes test.bb
pass1 - making usageList (1 chroms): 1 millis
Error line 1 of test_bb.BED: expecting 187185 elements in expIds list (bed 
field 14)


2.       In the regular BED format, it seems that "///" (slashes) are forbidden 
in the 4 column (name) if one would like to transform such file into bigBed 
format.

For example, I get this error:



./bedToBigBed test_bb_no_details.BED hg19.chrom.sizes test.bb

pass1 - making usageList (1 chroms): 1 millis

Expecting 12 words line 514 of test_bb_no_details.BED got 14



An example of an input of BED detail format for the bedToBigBed function (the 
14th column is empty, but it fails also if it is not) can be downloaded from:



http://www.weizmann.ac.il/complex/compphys/software/Amit/primers/human/tmp/test_bb.BED



When discarding the 13th and 14th columns so that the file is a regular BED 
file and removing lines with /// (slashes), the function bedToBigBed seems to 
work  fine.





Thanks a lot,



            Assif Yitzhaky

Weizmann Institute of Science








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