Relevant? Old news? Should be lots of nitrate and sulfate in the Arctic, at 
least in the ocean.  Not sure if possible N2O or NO2 production is a good idea, 
though.  - Greg

Nature 440, 918-921 (13 April 2006) | doi:10.1038/nature04617; Received 30 
November 2005; Accepted 2 February 2006

A microbial consortium couples anaerobic methane oxidation to denitrification

Ashna A. Raghoebarsing1, Arjan Pol1, Katinka T. van de Pas-Schoonen1, Alfons J. 
P. Smolders2, Katharina F. Ettwig1, W. Irene C. Rijpstra3, Stefan Schouten3, 
Jaap S. Sinninghe Damsté3, Huub J. M. Op den Camp1, Mike S. M. Jetten1 & Marc 
Strous1

Department of Microbiology, and
Department of Aquatic Ecology and Environmental Biology, Institute for Water 
and Wetland Research, Radboud University Nijmegen, Toernooiveld 1, 6525 ED 
Nijmegen, The Netherlands
Royal Netherlands Institute for Sea Research (NIOZ), Department of Marine 
Biogeochemistry and Toxicology, PO Box 59, 1790 AB Den Burg, The Netherlands
Correspondence to: Marc Strous1 Correspondence and requests for materials 
should be addressed to M.S. (Email: [email protected]). The 16S rRNA gene 
sequences have been deposited in GenBank under accession numbers DQ369741 
(archaeal sequence) and DQ369742 (bacterial sequence).

Top of pageAbstract
Modern agriculture has accelerated biological methane and nitrogen cycling on a 
global scale1, 2. Freshwater sediments often receive increased downward fluxes 
of nitrate from agricultural runoff and upward fluxes of methane generated by 
anaerobic decomposition3. In theory, prokaryotes should be capable of using 
nitrate to oxidize methane anaerobically, but such organisms have neither been 
observed in nature nor isolated in the laboratory4, 5, 6, 7, 8. Microbial 
oxidation of methane is thus believed to proceed only with oxygen or sulphate9, 
10. Here we show that the direct, anaerobic oxidation of methane coupled to 
denitrification of nitrate is possible. A microbial consortium, enriched from 
anoxic sediments, oxidized methane to carbon dioxide coupled to denitrification 
in the complete absence of oxygen. This consortium consisted of two 
microorganisms, a bacterium representing a phylum without any cultured species 
and an archaeon distantly related to marine methanotrophic Archaea. The 
detection of relatives of these prokaryotes in different freshwater ecosystems 
worldwide11, 12, 13, 14 indicates that the reaction presented here may make a 
substantial contribution to biological methane and nitrogen cycles.

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