Thanks for the update. But your bug is a little different from the one 
described below. Unlike C. metallidurans, the taxa reported by Kellermann are 
an anaerobic, chemoautortrophic Archea which use CH4 in dissimilatory mode as 
their energy source, not as a carbon source - so technically they're not 
methanotrophs. They use SO4 as the methane oxidant, not O2, unlike your 
bacteria.
Anyway, good luck with Arctic methane mitigation and keep us abreast of your 
progress. I might get behind the XL pipeline if it were extended to transport 
the fuel you will be producing.
Greg



>________________________________
> From: Michael Hayes <[email protected]>
>To: [email protected] 
>Sent: Tuesday, August 13, 2013 3:42 PM
>Subject: [geo] Re: Coupled CH4 and CO2 Mitigation?
> 
>
>
>Greg et al.,
> 
>A few weeks ago, I explored the use of Cupriavidus metalliduranswith the 
>Arctic methane groups. Insulated bioreactors would make this species useful in 
>the Arctic ebullition fields. Also, I believe non Arctic marine based systems, 
>such as OMEGA, could exploit this species for a co CO2/CH4 capture.
>
>Below is the text of the exploritory email:
> 
>"It may be possible to construct a continuous incubator for the production of 
>Cupriavidus metallidurans  which is a feed stock for Polyhydroxybutyrate. This 
>may help partially address the critical issue of Arctic methane releases in 
>areas of high ebullition.
>Of the many things I like about C. metallidurans, it is non pathogenic and is 
>a lithotroph. The first makes working with it easy and the second makes it 
>sympathetic to the use of olivine as the mineral source. 
>It is a mesophile. However, an incubator can compensate for Arctic 
>temperatures. 
>Here is the article which started me down this line of thought:How 
>Methane-Sourced Polymers Could Save the World.
>In past posts concerning concepts relitive to the Arctic Methane Tipping Point 
>and Arctic Sea Ice Loss, I've proposed creating multi-use Arctic sea platforms 
>using Shaf Downwellers  as the main structure. In the proposal I mentioned the 
>possibility of incorporating wave energy conversion through in-flow turbines 
>within the down flow, water spraying for ice enhancement and aerobic 
>methanotroph continuous incubators to help reduce desolved methane in the sea 
>water.
>The use of C. metallidurans continuous incubators over the high ebullition 
>methane fields of the ESAS, to produce feed stock for polyhydroxybutyrate 
>production, seems to make sense to me and they can be fitted into the overall 
>design.
>This type of mariculture may help offset the cost of Arctic methane 
>mitigation. 
>If you have time, please let me know if I've failed to properly connect the 
>dots on this idea. I'll put some work into the technical side to help show 
>what a 'continuous incubator' mounted to a Shaf Downweller may look like.".
> 
>Best,
> 
>Michael   
>
>On Tuesday, August 13, 2013 10:01:07 AM UTC-7, Greg Rau wrote:
>Sorry if this is old news, but in cleaning out my in box I came across this 
>interesting 2012 paper – anaerobic methane oxidation also consumes CO2.  So 
>with a bit of biogeoengineering we can pro-actively mitigate CH4 and CO2 
>simultaneously, +/- take the lipid-rich biomass to produce biofuels, 
>supplanting fossil sources???
>>Greg
>>
>>
>>
>>
>>
>>
>>Autotrophy as a predominant mode of carbon fixation in anaerobic 
>>methane-oxidizing microbial communities      1. Matthias Y. 
>>Kellermanna,1,2,3, 
>>      2. Gunter Wegenerb,c,1, 
>>      3. Marcus Elverta, 
>>      4. Marcos Yukio Yoshinagaa, 
>>      5. Yu-Shih Lina, 
>>      6. Thomas Hollerc, 
>>      7. Xavier Prieto Mollara, 
>>      8. Katrin Knittelc, and 
>>      9. Kai-Uwe Hinrichsa
>>+ Author Affiliations
>>1.     aOrganic Geochemistry Group, MARUM-Center for Marine Environmental 
>>Sciences and Department of Geosciences, University of Bremen, D-28359 Bremen, 
>>Germany; 
>>2.     bAlfred Wegener Institute for Polar and Marine Research, Research 
>>Group for Deep Sea Ecology and Technology, D-27515 Bremerhaven, Germany; and 
>>3.     cMax Planck Institute for Marine Microbiology, D-28359 Bremen, Germany 
>>1.     Edited by Donald E. Canfield, University of Southern Denmark, Odense 
>>M, Denmark, and approved October 5, 2012 (received for review May 24, 2012) 
>> 
>>Next Section
>>Abstract
>>The methane-rich, hydrothermally heated sediments of the Guaymas Basin are 
>>inhabited by thermophilic microorganisms, including anaerobic 
>>methane-oxidizing archaea (mainly ANME-1) and sulfate-reducing bacteria 
>>(e.g., HotSeep-1 cluster). We studied the microbial carbon flow in ANME-1/ 
>>HotSeep-1 enrichments in stable-isotope–probing experiments with and without 
>>methane. The relative incorporation of 13C from either dissolved inorganic 
>>carbon or methane into lipids revealed that methane-oxidizing archaea 
>>assimilated primarily inorganic carbon. This assimilation is strongly 
>>accelerated in the presence of methane. Experiments with simultaneous 
>>amendments of both 13C-labeled dissolved inorganic carbon and deuterated 
>>water provided further insights into production rates of individual lipids 
>>derived from members of the methane-oxidizing community as well as their 
>>carbon sources used for lipid biosynthesis. In the presence of methane, all 
>>prominent lipids
 carried a dual isotopic signal indicative of their origin from primarily 
autotrophic microbes. In the absence of methane, archaeal lipid production 
ceased and bacterial lipid production dropped by 90%; the lipids produced by 
the residual fraction of the metabolically active bacterial community 
predominantly carried a heterotrophic signal. Collectively our results strongly 
suggest that the studied ANME-1 archaea oxidize methane but assimilate 
inorganic carbon and should thus be classified as methane-oxidizing 
chemoorganoautotrophs. 
>> 
>> 
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