hi gmx users, i am new to gromacs , i am doing membrane protein simulations. My popc bilayer contain 128 molecules and protein contain 59 aminoacid residues, both systems i have simulated for 1ns . Now i want insert my protein into membrane. I have searched alot in gmxusers for how to insert protein into membrane and found that some are used make_hole.pl program, some of them have used genbox insertion command, The above mentioned two ways which one i have to follow, can anyone tell detail information. Thanks in advance.
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