shahrbanoo karbalaee wrote:
Dear
thanks  for  your answer. I did as you tell me  about using    capital
letter .about charge  I guess  it related to tfe and protonated  amino
acid.after   change  it  I  still have  this warning.


Then it's not case sensitivity, but what Mark told you is still right. The warning is there to alert people who couple (for example) ions separately. If your protein just happens to be small, then you will see the warning. It is not necessarily a problem.

-Justin

best

--
========================================

Justin A. Lemkul
Graduate Research Assistant
Department of Biochemistry
Virginia Tech
Blacksburg, VA
jalemkul[at]vt.edu | (540) 231-9080
http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin

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