Justin A. Lemkul wrote:
shahid nayeem wrote:
Hi Justin
Thanks a lot. What is the purpose of adding 100mM NaCl. Is it
mimicking physiological condition.
More of a hybrid of physiological and in vitro conditions. Please see
the referenced paper for more details.
...and again, please don't necessarily conclude that just because someone did
this for a tutorial that you should inherently be doing it for your system. The
tutorial is but one example of a workflow, derived from my own specific work.
Construct a model that is most appropriate to your purposes.
-Justin
--
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Justin A. Lemkul
Ph.D. Candidate
ICTAS Doctoral Scholar
MILES-IGERT Trainee
Department of Biochemistry
Virginia Tech
Blacksburg, VA
jalemkul[at]vt.edu | (540) 231-9080
http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin
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