Hi Shahid, You should try homology modeling software, like Modeller.
Cheers, Tsjerk On Aug 30, 2012 7:41 AM, "shahid nayeem" <[email protected]> wrote: Dear all Please suggest me a tool with which I can generate insertion mutants from a .pdb file. I want to insert new 10-15 aa sequence of AA in between an existing .pdb file. The tool should write a new .pdb file with altered coordinates after insertion of new sequence and minimization of the structure. shahid -- gmx-users mailing list [email protected] http://lists.gromacs.org/mailman/listinfo/gmx-users * Please search the archive at http://www.gromacs.org/Support/Mailing_Lists/Search before posting! * Please don't post (un)subscribe requests to the list. Use the www interface or send it to [email protected]. * Can't post? Read http://www.gromacs.org/Support/Mailing_Lists -- gmx-users mailing list [email protected] http://lists.gromacs.org/mailman/listinfo/gmx-users * Please search the archive at http://www.gromacs.org/Support/Mailing_Lists/Search before posting! * Please don't post (un)subscribe requests to the list. Use the www interface or send it to [email protected]. * Can't post? Read http://www.gromacs.org/Support/Mailing_Lists

