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Evaluation of a novel, two component, two step AFB cold staining method

Tripathi DG, Desai MW, Mesquita AM
Tulip Diagnostics (P) Ltd. Gitanjali, Tupil Block, Dr. Antonio Do Rego
Bagh, Bambolim complex P.O., Bambolim, Goa - 403 202, India

Correspondence Address:
Tulip Diagnostics (P) Ltd. Gitanjali, Tupil Block, Dr. Antonio Do Rego
Bagh, Bambolim complex P.O., Bambolim, Goa - 403 202, India

Abstract

AFB smear is a clinically useful and a cost effective test for
screening of sputum samples for Mycobacterium tuberculosis. Routinely,
Ziehl Neelsen stain, using the traditional hot staining procedure is
the mainstay of sputum smear AFB tests. We have evaluated a novel two
components, two step cold AFB stain to establish its sensitivity,
simplicity and reproducibility as compared to the traditional 'ZN Hot'
stain still regarded as the standard AFB stain in routine laboratory
practice. This study was conducted over a period of four months at the
TB Hospital, Taleigao Goa. Comparatively the two components, two step
AFB stain was easy to use, time, labour and cost saving. It gave
reproducible results during the study period. The most important
characteris tics were that it nearly doubled (~91%) the detection rate
of AFB positive sputum in our hospital.

How to cite this article:
Tripathi DG, Desai MW, Mesquita AM. Evaluation of a novel, two
component, two step AFB cold staining method. Indian J Med Microbiol
2001;19:163-165

How to cite this URL:
Tripathi DG, Desai MW, Mesquita AM. Evaluation of a novel, two
component, two step AFB cold staining method. Indian J Med Microbiol
[serial online] 2001 [cited 2006 Aug 30];19:163-165. Available from:
http://www.ijmm.org/article.asp?issn=0255-0857;year=2001;volume=19;issue=3;spage=163;epage=165;aulast=Tripathi

Thanks to the Directory of Open Access Journals http://www.doaj.org in
Lund, Sweden.
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Frederick Noronha http://fn.goa-india.org  9822122436 +91-832-240-9490
http://fredericknoronha.wordpress.com [EMAIL PROTECTED]
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