That does seem a high.  Does it also say that a new instance was started for 
this request?  That would make it less surprising.

Are you storing/getting all the entities in one db call?  That's the most 
straightforward way to speed up requests.  There's some overhead to 
serializing and deserializing lists, but I don't think it should be that 
much.  

Also, consider whether some of this data doesn't need indexes.  I think 
writing indexes for a list can be time consuming.

AppStats is a magnificent boon that will really help you to see where the 
time is going.
http://code.google.com/appengine/docs/python/tools/appstats.html

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