this problem persists about one hour, below is my error log, thanks for your 
help:

*Application: bio-enzyme-v2; version: 1362-65dfd0f2ce44-1 (was: 
736-207d478f1490-1)*
*Host: appengine.google.com*
*
*
*Starting update of app: bio-enzyme-v2, version: 1362-65dfd0f2ce44-1*
*Scanning files on local disk.*
*Scanned 500 files.*
*Scanned 1000 files.*
*Scanned 1500 files.*
*Scanned 2000 files.*
*Scanned 2500 files.*
*Cloning 795 static files.*
*Cloned 100 files.*
*Cloned 200 files.*
*Cloned 300 files.*
*Cloned 400 files.*
*Cloned 500 files.*
*Cloned 600 files.*
*Cloned 700 files.*
*Cloning 1739 application files.*
*Cloned 100 files.*
*Cloned 200 files.*
*Rolling back the update.*
*Error 500: --- begin server output ---*
*
*
*<html><head>*
*<meta http-equiv="content-type" content="text/html;charset=utf-8">*
*<title>500 Server Error</title>*
*</head>*
*<body text=#000000 bgcolor=#ffffff>*
*<h1>Error: Server Error</h1>*
*<h2>The server encountered an error and could not complete your 
request.<p>If the problem persists, please <A 
HREF="http://code.google.com/appengine/community.html";>report</A> your 
problem and mention this error message and the query that caused it.</h2>*
*<h2></h2>*
*</body></html>*
*--- end server output ---*

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