On 10/15/14 2:19 PM, Diogo Martins de Sá wrote:
Fellow users,


I have read several emails exchanged in this discussion list where
g_dist is presented as a better choice for observing salt bridges in the
trajectory. And I'm totally inclined to agree with that, but that would
be the case where you already know which are the SBs present in the
system. If one has this information, he could easily create groups of
individual residues (which would actually contain just the ionazable
atoms and their hydrogens, depricating the carbons and other atoms) and
observe how the salt bridges behave.


My question is concerning the situation where you still don't know which
are the salt bridges in the system and, after that, which are the ones
you should take a closer look (I think the obvious approach would be to
search literature, but that is not always successful).


Justin Lemkul has mentioned, in two diferent discussions, using g_dist
to "track persistence of interactions" and "looping calculations" to
address the situation I've just mentioned.


Could someone elaborate on those? I am confused as to what they actually
mean and how to proceed (especially in the case of "looping
calculations").


Likely I was referring to looping over calls to g_dist (in the future, please link to any previous discussions - it's hard for me to keep track of all of the thousands of messages I've sent :)

One can certainly use g_saltbr to look at positive-negative interactions en masse, but it's a fairly "dumb" program, in that it considers a huge amount of groups that don't matter. You can weed through all of that to find things that might be of interest.

-Justin

--
==================================================

Justin A. Lemkul, Ph.D.
Ruth L. Kirschstein NRSA Postdoctoral Fellow

Department of Pharmaceutical Sciences
School of Pharmacy
Health Sciences Facility II, Room 629
University of Maryland, Baltimore
20 Penn St.
Baltimore, MD 21201

[email protected] | (410) 706-7441
http://mackerell.umaryland.edu/~jalemkul

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