Dear All,
 
By H++ and reduce we can have the input PDB for pdb2gmx have the initial 
corrected protonated states for some residues, for example, HIS, ARG, GLU and 
terminal residues. However biologically speaking with the change of the protein 
conformation, the protonated states of the above residues can be changed.
 
Will you please introduce to me how GROMACS deal with the possible protonation 
state change during the MD process?
 
Brett
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