On 6/8/15 9:19 AM, Zhang, Cheng wrote:
Dear Gromacs expert,

I was trying to extend my simulations. Relevant files can be found at

https://copy.com/c8dDMnC0df2gbig9


To prepare the "md_0_1.tpr" file, I set the following in the "md_50ns.mdp" so 
that it can run 50 ns in total.


nsteps = 25000000

dt    = 0.002


Then I submit the same job for 5 times with the following command:


gerun mdrun_mpi -deffnm md_0_1.tpr -cpi md_0_1.cpt -maxh 30 -append

(Each of the 5 jobs would be hold until the previous one had finished.)


However, as I check the 5 xtc files (e.g. RMSD):


#md_0_1.tpr.xtc.1#

#md_0_1.tpr.xtc.2#

#md_0_1.tpr.xtc.3#

#md_0_1.tpr.xtc.4#

md_0_1.tpr.xtc


all of them start the simulation from 0 to 10000 ps with different 
fluctuations. As I understand, they all start from the beginning instead of 
extending the simulation based on its previous one.


So can I ask if I have done something incorrect?


If you're not modifying the contents of the .tpr file to indicate that you want to extend the simulation, mdrun is just going to run the same simulation. It's doing what you have told it to do. You need to invoke gmx convert-tpr (previously called tpbconv). See the wiki:

http://www.gromacs.org/Documentation/How-tos/Extending_Simulations#Version_4_and_Newer

-Justin

--
==================================================

Justin A. Lemkul, Ph.D.
Ruth L. Kirschstein NRSA Postdoctoral Fellow

Department of Pharmaceutical Sciences
School of Pharmacy
Health Sciences Facility II, Room 629
University of Maryland, Baltimore
20 Penn St.
Baltimore, MD 21201

[email protected] | (410) 706-7441
http://mackerell.umaryland.edu/~jalemkul

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