On Wed, 28 Dec 2016 17:24:08 +0100
Alex <[email protected]> wrote:

> Thank for your response.
> 
> You mean for the TI analysis, now problem if one .xvg file (e.g.
> case.3.xvg) has just 15 columns while another .xvg file (e.g.
> case.18.xvg) has 20 columns? and still the "alchemical analysis
> package" could be used to calculate the free energy change via TI
> method?

I suppose you mean David Mobley's tool.  Yes, it can do that.  You can
easily check that yourself though.
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