Hi Dr.Lemkul Thank you very much for your answer
I get it now, before your answer I run system with -maxwarn 1, I saw trajectory in vmd , my drug molecule break in middle of run and then corrected, is it wrong? has it occured because using maxwran? is vmd an appropriate tool for detect trajectory? I alaways after run, check trajectory with vmd to sure in time of simulation all structure were complete. I will do your advise, and I will run with Benedsen probably it will solve my problem Thank you very much Regards Azadeh -- This email was Anti Virus checked by Security Gateway. -- Gromacs Users mailing list * Please search the archive at http://www.gromacs.org/Support/Mailing_Lists/GMX-Users_List before posting! * Can't post? Read http://www.gromacs.org/Support/Mailing_Lists * For (un)subscribe requests visit https://maillist.sys.kth.se/mailman/listinfo/gromacs.org_gmx-users or send a mail to gmx-users-requ...@gromacs.org.