Hi

In the surf2surf command, the input should be a surf.gii file and the output a 
gii file (without the “surf”), which will combine information from both the 
surf.gii file (the actual surface)  and the func.gii file (which contains your 
ROI).

You can visualise your ROI in either workbench or fslview.

In workbench, simply load the surf.gii and the func.gii.

In FSL, you need to first project the output of surf2surf onto voxels:
surf2volume L.frontalpole.gii SomeVolumeInMNISpace.nii.gz output.nii.gz caret
fslview output.nii.gz

Cheers
Saad






On 16 Jul 2014, at 05:37, Patricia Hong Phi Tran 
<[email protected]<mailto:[email protected]>> wrote:

Hi,

We are trying to run surface-to-surface tractography using probtrackx and 
generate whole-brain connectomes with the HCP data but have run into a number 
of problems.
We attempted to follow the wiki but label2surf only works with Freesurfer label 
files, not the GIFTI ones included.

Therefore;
1) We used wb_command -gifti-label-to-roi with the L.aparc.32k_fs_LR.label 
GIFTI file to extract the frontal and temporal pole into respective GIFTI 
labels (e.g. lh.frontalpole.func.gii and lh.temporalpole.func.gii). We are not 
sure that this worked as we cannot visualise the files in either the workbench 
or FSL due to incompatibility issues.
wb_command -gifti-label-to-roi 
100307/MNINonLinear/fsaverage_LR32k/100307.L.aparc.32k_fs_LR.label.gii  
/100307/L.frontalpole.func.gii -name L_frontalpole

2) Then we attempted to generate surface/label files from these individual 
label files using surf2surf (e.g. lh.frontalpole.func.gii --> 
lh.frontalpole.surf.gii)
surf2surf -i L.frontalpole.func.gii -o L.frontalpole.surf.gii 
--values=L.frontalpole.func.gii

3) Fed in both the frontal and temporal pole .surf.gii files to probtrackx2 
using the "multiple masks" option in the GUI, selecting Matrix1 as an option 
with otherwise standard settings.

4) It ran for ~66,000 seconds and then 'finished' with the following error 
message (attached) and the matrix output consisted of 0s, indicating that no 
tracts appeared to have been run at all.

It ultimately seems to be a problem with the GIFTI label extraction and 
conversion but we are not sure what the fix is.

Thanks in advance,

- Patricia

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<Screen Shot 2014-07-16 at 9.31.51 AM.jpg>

--
Saad Jbabdi
University of Oxford, FMRIB Centre

JR Hospital, Headington, OX3 9DU, UK
(+44)1865-222466  (fax 717)
www.ndcn.ox.ac.uk/team/researchers/saad-jbabdi<http://www.ndcn.ox.ac.uk/team/researchers/saad-jbabdi>


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