Hi Donna,

That is correct that we want the source parcellation mapped on the subjects
surfaces. I believe we are wanting it in MNINonlinear stereotaxic space.

Should I be using the fs_LR standard mesh then?

With the mri_label2vol command I should be only producing a single output
volume. I now input the annotation file into this command, bypassing the
need for labels (the computer I am using also has trouble loading in all of
the labels).

I have a pre-existing script that was written in my lab to create random
parcellations and I am attempting to adapt that. So I do the above and I
will get a label volume for each hemisphere. Then these two label volumes
are combined into a single image. Subcortical parcellations are then added
to make a whole brain parcellation image which we then use to make
connectomes.

Sorry if anything is unclear or not elaborated on enough, I am very new to
all of this.

Regards,

Stuart

On 27 May 2015 at 07:01, Donna Dierker <[email protected]> wrote:

> Hi Stuart,
>
> I have fewer answers than questions, but it seems like at the end of this,
> you want your source (fsaverage) parcellation mapped onto the HCP subjects'
> surfaces, and then projected from surface to volume (subject's orig/magnet
> space or MNINonlinear stereotaxic space?).
>
> The HCP pipeline uses the Freesurfer ?h.sphere.reg to get surface data
> onto fs_LR mesh:
>
> lh: freesurfer_native_mesh -> fsaverage.left -> fs_LR (32k or 164k) mesh
> rh: freesurfer_native_mesh -> fsaverage.right -> fs_LR (32k or 164k) mesh
>
> Note that fsaverage left and right hem meshes are not in register with one
> another (across hemispheres -- vertex i on the left is probably nowhere
> near vertex i on the right hem).  In the fs_LR standard mesh, left and
> right hems are in register with one another.
>
> It sounds like you have an annotation on the fsaverage mesh.  It seems in
> principle possible to get your annotation, which is a single, atlas-like
> parcellation that will be used across HCP subjects, onto the fs_LR mesh,
> and then you could use the subjects' fs_LR white, midthickness, or pial
> surface to project the annotation/label to volume somehow, but I don't know
> how those freesurfer commands work or what they need.  You can mris_convert
> from GIFTI to Freesurfer format, if needed.
>
> It might help to zoom out a bit more, though:  What do you plan to do with
> the resulting individual label volumes?
>
> Donna
>
>
> On May 24, 2015, at 8:45 PM, Stuart Oldham <[email protected]>
> wrote:
>
> > Hi all,
> >
> > I am trying to create a random parcellation for the HCP data. I have
> created the parcellations first using the fsaverage subject and am trying
> to map these onto the HCP subjects.
> >
> > First I have map the annotation from the source (fsaverage) to the
> subject using mri_surf2surf (for each hemisphere individually). Usually
> this just uses a sphere.reg file but the HCP data doesn't have the file in
> this format. Is the {subject}.{hemisphere}.sphere.reg.native.surf.gii
> (located under MNINonLinear/Native) the file I should be using here?
> >
> > Next I turn the annotations into labels using mri_annotation2label.
> Which surface file do I need to specify it to run (is it the same as above)?
> >
> > Then I run mri_label2vols. Again which surface file should I be
> inputting? Should it now be the
> {subject}.{hemisphere}.white.native.surf.gii? Also is there a way with this
> command I can specify it to find a thickness file (i.e.
> {subject}.{hemisphere}.thickness.native.surf.gii) as by default it is
> looking for an lh or rh .thickness file.
> >
> > Thanks in advance!
> >
> > _______________________________________________
> > HCP-Users mailing list
> > [email protected]
> > http://lists.humanconnectome.org/mailman/listinfo/hcp-users
> >
>
>

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