We face the block and put it in a dish with rapid decal solution. For tough blocks like uterus we soak them in a solution of water, ammonia(which is great for bloody tissue) and liquid soap.
-----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Laurie Reilly Sent: Friday, October 03, 2008 7:58 PM To: 'Piero Nelva'; [email protected] Subject: RE: [Histonet] Does anyone have any ideas on the use of softenerondecal blocks. Does this help 'soften' them? If so how? Floating the trimmed/faced block on the waterbath for 2 minutes helps. Then put them on wet ice to cool. Regards, Laurie. Mr. Laurie REILLY Histopathology School of Veterinary and Biomedical Sciences James Cook University Townsville Qld. 4811 Australia. Phone 07 4781 4468 -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Piero Nelva Sent: Friday, 3 October 2008 7:16 PM To: [email protected] Subject: Re: [Histonet] Does anyone have any ideas on the use of softener ondecal blocks. Does this help 'soften' them? If so how? Hi Sarah I don't use a softener as such, but have had good results putting trimmed blocks on a water soaked tissue on the bench for 30 min or so just before cooling and sectioning. I find it is more effective than an ice bath at softening tissues. REgards Piero Nelva Anatomical Pathology Monash Medical Centre Australia ----- Original Message ----- From: "SARAH REEVES" <[EMAIL PROTECTED]> To: <[email protected]> Sent: Friday, October 03, 2008 12:34 AM Subject: [Histonet] Does anyone have any ideas on the use of softener on decal blocks. Does this help 'soften' them? If so how? Does anyone have any ideas on the use of softener on decal blocks. Does this help 'soften' them? If so how? Thanks in advance Sarah ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of East Kent Hospitals University NHS Trust. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you have received this email in error please notify the system manager at the following email address: [EMAIL PROTECTED] www.kentandmedway.nhs.uk This footnote also confirms that although this email message has been swept by MIMEsweeper for the presence of computer viruses, it is strongly recommended that you carry out your own virus scan of this message and any attachments. www.mimesweeper.com ********************************************************************** _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ ---- ---- No virus found in this incoming message. Checked by AVG - http://www.avg.com Version: 8.0.173 / Virus Database: 270.7.5/1704 - Release Date: 10/2/2008 9:35 PM _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
